Coding

Part:BBa_K3582301:Design

Designed by: Jatin Bedi, Avadhoot Jadhav   Group: iGEM20_IISER-Pune-India   (2020-10-22)


Basigin protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 67
    Illegal NgoMIV site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 358
    Illegal SapI.rc site found at 330
    Illegal SapI.rc site found at 747


Design Notes

  • 1] RFC10 criteria satisfied
  • 2]Strep Ⅱ tag gene added for purification
  • 3] Codon optimized with E-coli K-12 strain



Source

Reverse Translation.

References